Application of Label-Free Absolute Quantitative Proteomics in Human Gingival Crevicular Fluid by LC/MSE(Gingival Exudatome)

Application of label-free absolute quantitative proteomics in human gingival crevicular fluid by LC/MS E (gingival exudatome).

Periodontal disease is perhaps the most common infectious disease in humans. Gingival crevicular fluid (GCF) is a local inflammatory exudate of the periodontal tissues. Its composition greatly varies between health and periodontal disease. GCF collection is rapid and noninvasive, but previous approaches aiming to analyze its composition have mainly involved single protein biomarkers. The aim of...

Gingival Crevicular Fluid Biomarkers

Material and Method: Thirty patients were allocated into 3 groups: 10 were treated with scaling and root planing (SRP) only; 10 were treated with SRP and received adjunctive local metronidazole; 10 were treated with SRP and received adjunctive systemic metronidazole. Periodontal clinical parameters including plaque index, sulcus bleeding index, probing depth and relative attachment level were r...

Human Gingival Crevicular Fluids (GCF) Proteomics: An Overview

Like other fluids of the human body, a gingival crevicular fluid (GCF) contains proteins, a diverse population of cells, desquamated epithelial cells, and bacteria from adjacent plaque. Proteomic tools have revolutionized the characterization of proteins and peptides and the detection of early disease changes in the human body. Gingival crevicular fluids (GCFs) are a very specific oral cavity f...

Hydroxyproline titers in gingival crevicular fluid.

Mass Spectrometry-Based Label-Free Quantitative Proteomics

In order to study the differential protein expression in complex biological samples, strategies for rapid, highly reproducible and accurate quantification are necessary. Isotope labeling and fluorescent labeling techniques have been widely used in quantitative proteomics research. However, researchers are increasingly turning to label-free shotgun proteomics techniques for faster, cleaner, and ...